Plasmid transfection calculator for lentivirus production in HEK293T cells Plasmid transfection calculator Plasmid transfection calculator Culture vessel Select T75 flask 6-well plate well 24-well plate well Number of vessels (1-24) DNA plasmid Concentration (ng/μL) Mass ratio Mass (μg) Volume (μL) Transgene plasmid (i.e., pE) 3 Packaging plasmid (i.e., psPAX2) 2 Envelope plasmid (i.e., pMD2) 1 Calculate Reset
